A specific domain of the Chikungunya virus E2 protein regulates particle formation in human cells: implications for alphavirus vaccine design
W Akahata, GJ Nabel - Journal of virology, 2012 - Am Soc Microbiol
W Akahata, GJ Nabel
Journal of virology, 2012•Am Soc MicrobiolVirus-like particles (VLPs) can be generated from Chikungunya virus (CHIKV), but different
strains yield variable quantities of particles. Here, we define the genetic basis for these
differences and show that amino acid 234 in E2 substantially affects VLP production. This
site is located within the acid-sensitive region (ASR) known to initiate a major conformational
change in E1/E2. Selected other mutations in the ASR, or changes in pH, also increased
VLP yield. These results demonstrate that the ASR of E2 plays an important role in …
strains yield variable quantities of particles. Here, we define the genetic basis for these
differences and show that amino acid 234 in E2 substantially affects VLP production. This
site is located within the acid-sensitive region (ASR) known to initiate a major conformational
change in E1/E2. Selected other mutations in the ASR, or changes in pH, also increased
VLP yield. These results demonstrate that the ASR of E2 plays an important role in …
Abstract
Virus-like particles (VLPs) can be generated from Chikungunya virus (CHIKV), but different strains yield variable quantities of particles. Here, we define the genetic basis for these differences and show that amino acid 234 in E2 substantially affects VLP production. This site is located within the acid-sensitive region (ASR) known to initiate a major conformational change in E1/E2. Selected other mutations in the ASR, or changes in pH, also increased VLP yield. These results demonstrate that the ASR of E2 plays an important role in regulating particle generation.
American Society for Microbiology