[HTML][HTML] Structural and functional analysis of the recombinant G domain of the laminin α4 chain and its proteolytic processing in tissues
JF Talts, T Sasaki, N Miosge, W Göhring… - Journal of Biological …, 2000 - ASBMB
The C-terminal G domains of laminin α chains have been implicated in various cellular and
other interactions. The G domain of the α4 chain was now produced in transfected
mammalian cells as two tandem arrays of LG modules, α4LG1–3 and α4LG4–5. The
recombinant fragments were shown to fold into globular structures and could be
distinguished by specific antibodies. Both fragments were able to bind to heparin, sulfatides,
and the microfibrillar fibulin-1 and fibulin-2. They were, however, poor substrates for cell …
other interactions. The G domain of the α4 chain was now produced in transfected
mammalian cells as two tandem arrays of LG modules, α4LG1–3 and α4LG4–5. The
recombinant fragments were shown to fold into globular structures and could be
distinguished by specific antibodies. Both fragments were able to bind to heparin, sulfatides,
and the microfibrillar fibulin-1 and fibulin-2. They were, however, poor substrates for cell …