[HTML][HTML] C5a delays apoptosis of human neutrophils by a phosphatidylinositol 3-kinase-signaling pathway
MC Perianayagam, VS Balakrishnan, AJ King… - Kidney international, 2002 - Elsevier
MC Perianayagam, VS Balakrishnan, AJ King, BJG Pereira, BL Jaber
Kidney international, 2002•ElsevierC5a delays apoptosis of human neutrophils by a phosphatidylinositol 3-kinase-signaling
pathway. Background Studies have shown that survival factors including cytokines and
growth factors delay apoptosis of human neutrophils via induction of the phosphatidylinositol-
3 kinase (PI 3-K)/Akt pathway. In the present study, we explored whether complement
fragment C5a has a modulatory effect on neutrophil apoptosis through this signaling
pathway. Methods Human neutrophils were isolated and treated with C5a for up to 24 hours …
pathway. Background Studies have shown that survival factors including cytokines and
growth factors delay apoptosis of human neutrophils via induction of the phosphatidylinositol-
3 kinase (PI 3-K)/Akt pathway. In the present study, we explored whether complement
fragment C5a has a modulatory effect on neutrophil apoptosis through this signaling
pathway. Methods Human neutrophils were isolated and treated with C5a for up to 24 hours …
C5a delays apoptosis of human neutrophils by a phosphatidylinositol 3-kinase-signaling pathway.
Background
Studies have shown that survival factors including cytokines and growth factors delay apoptosis of human neutrophils via induction of the phosphatidylinositol-3 kinase (PI 3-K)/Akt pathway. In the present study, we explored whether complement fragment C5a has a modulatory effect on neutrophil apoptosis through this signaling pathway.
Methods
Human neutrophils were isolated and treated with C5a for up to 24 hours, with or without wortmannin, a PI 3-K inhibitor, and staurosporine, a caspase-9 activator. Apoptosis was quantified by flow cytometry, using propidium iodide nuclear staining, and confirmed by the detection of DNA fragmentation on gel electrophoresis. PI 3-K downstream signaling events were evaluated by measuring the expression of cytosolic total and phosphorylated Akt and Bad proteins by Western blot analyses, and caspase-9 activity.
Results
C5a inhibited neutrophil apoptosis in a dose- and time-dependent manner. The anti-apoptotic effects of C5a were markedly abrogated in the presence of wortmannin. Brief stimulation of neutrophils with C5a induced phosphorylation of Akt and Bad proteins through a PI 3-K–dependent pathway. Caspase-9 activity was minimal in C5a-treated cells, but markedly increased following PI 3-K inhibition by wortmannin. Finally, C5a reduced caspase-9 activity in staurosporine-treated cells.
Conclusions
This study demonstrates that C5a inhibits neutrophil apoptosis via a PI 3-K signaling pathway. This effect may be an important mechanism that improves cell survival and function in the inflammatory milieu.
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