New techniques for biopsy and culture of human olfactory epithelial neurons

F Féron, C Perry, JJ McGrath… - … of otolaryngology–head …, 1998 - jamanetwork.com
Objective To improve the success of culturing olfactory neurons from human nasal mucosa
by investigating the intranasal distribution of the olfactory epithelium and devising new
techniques for growing human olfactory epithelium in vitro. Design Ninety-seven biopsy
specimens were obtained from 33 individuals, aged 21 to 74 years, collected from 6 regions
of the nasal cavity. Each biopsy specimen was bisected, and 1 piece was processed for
immunohistochemistry or electron microscopy while the other piece was dissected further for …