Circadian rhythms and the effect of glucocorticoids on expression of the clock gene period1 in canine peripheral blood mononuclear cells
K Ohmori, S Nishikawa, K Oku, K Oida, Y Amagai… - The Veterinary …, 2013 - Elsevier
Circadian rhythms have a periodicity of approximately 24h and, in mammals, are regulated
by clock genes. In this study, expression profiles of clock genes (per1, per2, clock, bmal1
and cry1) were investigated over a single 24h period by real-time PCR in peripheral blood
mononuclear cells (PBMCs) of healthy dogs and canine PBMCs treated in vitro and in vivo
with glucocorticoids. Only per1 mRNA exhibited daily rhythms in canine PBMCs. Canine
PBMCs cultured with dexamethasone in vitro had dose-and time-dependent increases in …
by clock genes. In this study, expression profiles of clock genes (per1, per2, clock, bmal1
and cry1) were investigated over a single 24h period by real-time PCR in peripheral blood
mononuclear cells (PBMCs) of healthy dogs and canine PBMCs treated in vitro and in vivo
with glucocorticoids. Only per1 mRNA exhibited daily rhythms in canine PBMCs. Canine
PBMCs cultured with dexamethasone in vitro had dose-and time-dependent increases in …