[HTML][HTML] Inhibition of IκB kinase activity by sodium salicylate in vitro does not reflect its inhibitory mechanism in intact cells
D Alpert, J Vilček - Journal of Biological Chemistry, 2000 - ASBMB
Sodium salicylate inhibits activation of the transcription factor NF-κB by blocking the
phosphorylation and degradation of the NF-κB inhibitor IκBα. We previously demonstrated
that salicylate inhibits IκBα degradation induced by tumor necrosis factor (TNF) but not by
interleukin-1 (IL-1) and implicated p38 mitogen-activated protein kinase activation by
salicylate in the inhibition of TNF-induced IκBα phosphorylation. Both TNF and IL-1 rapidly
activate the IκB kinase (IKK) complex, containing the catalytic subunits IKKα and IKKβ, which …
phosphorylation and degradation of the NF-κB inhibitor IκBα. We previously demonstrated
that salicylate inhibits IκBα degradation induced by tumor necrosis factor (TNF) but not by
interleukin-1 (IL-1) and implicated p38 mitogen-activated protein kinase activation by
salicylate in the inhibition of TNF-induced IκBα phosphorylation. Both TNF and IL-1 rapidly
activate the IκB kinase (IKK) complex, containing the catalytic subunits IKKα and IKKβ, which …