Regional, developmental and interspecies expression of the four NMDAR2 subunits, examined using monoclonal antibodies
DJ Laurie, I Bartke, R Schoepfer, K Naujoks… - Molecular Brain …, 1997 - Elsevier
Mouse monoclonal antibodies were raised against bacterially expressed protein sequences
of the NR2A, NR2B, NR2C and NR2D subunits of the rat NMDA receptor. From immunoblots
of rat brain proteins, the apparent molecular weights of these subunits were 165, 170, 135
and 145 kDa, respectively. Proteins of similar masses were observed on immunoblots of
specifically transfected HEK293 cells. Deglycosylation with endoglycosidase F reduced the
mass of each endogenous NR2 subunit by≈ 10 kDa. In distribution studies, NR2A …
of the NR2A, NR2B, NR2C and NR2D subunits of the rat NMDA receptor. From immunoblots
of rat brain proteins, the apparent molecular weights of these subunits were 165, 170, 135
and 145 kDa, respectively. Proteins of similar masses were observed on immunoblots of
specifically transfected HEK293 cells. Deglycosylation with endoglycosidase F reduced the
mass of each endogenous NR2 subunit by≈ 10 kDa. In distribution studies, NR2A …