[HTML][HTML] Insulin-induced up-regulated uncoupling protein-1 expression is mediated by insulin receptor substrate 1 through the phosphatidylinositol 3-kinase/Akt …
AM Valverde, M Arribas, C Mur, P Navarro… - Journal of Biological …, 2003 - ASBMB
To investigate the role of insulin receptor substrate-1 (IRS-1) and its downstream signaling
in insulin-induced thermogenic differentiation of brown adipocytes, we have reconstituted
IRS-1-deficient fetal brown adipocytes (IRS-1−/−) with wild-type IRS-1 (IRS-1 wt). The lack of
IRS-1 resulted in the inability of insulin to induce IRS-1-associated phosphatidylinositol 3-
kinase (PI 3-kinase) activity and Akt phosphorylation in IRS-1−/− brown adipocytes. In
addition, these cells showed an impairment in activating α-Akt, β-Akt, and γ-Akt isoforms …
in insulin-induced thermogenic differentiation of brown adipocytes, we have reconstituted
IRS-1-deficient fetal brown adipocytes (IRS-1−/−) with wild-type IRS-1 (IRS-1 wt). The lack of
IRS-1 resulted in the inability of insulin to induce IRS-1-associated phosphatidylinositol 3-
kinase (PI 3-kinase) activity and Akt phosphorylation in IRS-1−/− brown adipocytes. In
addition, these cells showed an impairment in activating α-Akt, β-Akt, and γ-Akt isoforms …