Pyk2 is a member of the focal adhesion kinase (FAK) family, highly expressed in the central nervous system and haemopoietic cells. Although Pyk2 is homologous to FAK, its role in signaling pathways was shown to be distinct from that of FAK. We show here that Pyk2 is highly expressed in peritoneal IC‐21 macrophage and is tyrosine phosphorylated in response to cell attachment to fibronectin and fibrinogen. Upon IC‐21 cell adhesion, Pyk2 tyrosine phosphorylation is inhibited by blocking antibodies to the integrin subunits α_M and β₂. Furthermore, Pyk2 is rapidly tyrosine phosphorylated in response to ligation of β₂ integrins by antibodies. In migrating macrophages, Pyk2 localizes to perinuclear regions and to podosomes, where it is clustered with tyrosine phosphorylated proteins. Furthermore, in the podosomal ring structure, which surrounds the central actin core, Pyk2 co‐localizes with vinculin, talin, and paxillin. In the podosomes, Pyk2 also co‐localizes with the integrin α_Mβ₂. Lastly, reduction of Pyk2 expression in macrophages leads to inhibition of cell migration. We propose that Pyk2 is functionally linked to the formation of podosomes where it mediates the integrin‐cytoskeleton interface and regulates cell spreading and migration. Cell Motil. Cytoskeleton 47:174–188, 2000. © 2000 Wiley‐Liss, Inc.