INTRODUCTION: Flow cytometry allows the analysis of multiple antigens in a single tube at
a single cell level. We present a rapid and sensitive two tube flow cytometric protocol for the
detection of multiple platelet antigens and activation markers gated on a pure platelet
population. MATERIALS AND METHODS: The presence of platelet specific antigens was
analyzed in citrated whole blood of normal platelets and from patients diagnosed with
platelet abnormalities. Quiescent platelets as well as stimulated platelets were analyzed …