[HTML][HTML] Allogeneic T cells that express an anti-CD19 chimeric antigen receptor induce remissions of B-cell malignancies that progress after allogeneic hematopoietic …

JN Brudno, RPT Somerville, V Shi, JJ Rose… - Journal of clinical …, 2016 - ncbi.nlm.nih.gov
JN Brudno, RPT Somerville, V Shi, JJ Rose, DC Halverson, DH Fowler, JC Gea-Banacloche…
Journal of clinical oncology, 2016ncbi.nlm.nih.gov
Purpose Progressive malignancy is the leading cause of death after allogeneic
hematopoietic stem-cell transplantation (alloHSCT). After alloHSCT, B-cell malignancies
often are treated with unmanipulated donor lymphocyte infusions (DLIs) from the transplant
donor. DLIs frequently are not effective at eradicating malignancy and often cause graft-
versus-host disease, a potentially lethal immune response against normal recipient tissues.
Methods We conducted a clinical trial of allogeneic T cells genetically engineered to express …
Abstract
Purpose
Progressive malignancy is the leading cause of death after allogeneic hematopoietic stem-cell transplantation (alloHSCT). After alloHSCT, B-cell malignancies often are treated with unmanipulated donor lymphocyte infusions (DLIs) from the transplant donor. DLIs frequently are not effective at eradicating malignancy and often cause graft-versus-host disease, a potentially lethal immune response against normal recipient tissues.
Methods
We conducted a clinical trial of allogeneic T cells genetically engineered to express a chimeric antigen receptor (CAR) targeting the B-cell antigen CD19. Patients with B-cell malignancies that had progressed after alloHSCT received a single infusion of CAR T cells. No chemotherapy or other therapies were administered. The T cells were obtained from each recipient’s alloHSCT donor.
Results
Eight of 20 treated patients obtained remission, which included six complete remissions (CRs) and two partial remissions. The response rate was highest for acute lymphoblastic leukemia, with four of five patients obtaining minimal residual disease–negative CR. Responses also occurred in chronic lymphocytic leukemia and lymphoma. The longest ongoing CR was more than 30 months in a patient with chronic lymphocytic leukemia. New-onset acute graft-versus-host disease after CAR T-cell infusion developed in none of the patients. Toxicities included fever, tachycardia, and hypotension. Peak blood CAR T-cell levels were higher in patients who obtained remissions than in those who did not. Programmed cell death protein-1 expression was significantly elevated on CAR T cells after infusion. Presence of blood B cells before CAR T-cell infusion was associated with higher postinfusion CAR T-cell levels.
Conclusion
Allogeneic anti-CD19 CAR T cells can effectively treat B-cell malignancies that progress after alloHSCT. The findings point toward a future when antigen-specific T-cell therapies will play a central role in alloHSCT.
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