Clinical application of Sleeping Beauty and artificial antigen presenting cells to genetically modify T cells from peripheral and umbilical cord blood
MH Huls, MJ Figliola, MJ Dawson, S Olivares… - JoVE (Journal of …, 2013 - jove.com
The potency of clinical-grade T cells can be improved by combining gene therapy with
immunotherapy to engineer a biologic product with the potential for superior (i) recognition
of tumor-associated antigens (TAAs),(ii) persistence after infusion,(iii) potential for migration
to tumor sites, and (iv) ability to recycle effector functions within the tumor microenvironment.
Most approaches to genetic manipulation of T cells engineered for human application have
used retrovirus and lentivirus for the stable expression of CAR1-3. This approach, although …
immunotherapy to engineer a biologic product with the potential for superior (i) recognition
of tumor-associated antigens (TAAs),(ii) persistence after infusion,(iii) potential for migration
to tumor sites, and (iv) ability to recycle effector functions within the tumor microenvironment.
Most approaches to genetic manipulation of T cells engineered for human application have
used retrovirus and lentivirus for the stable expression of CAR1-3. This approach, although …