[HTML][HTML] Intracellular K+ suppresses the activation of apoptosis in lymphocytes
FM Hughes, CD Bortner, GD Purdy… - Journal of Biological …, 1997 - ASBMB
Little is known about the mechanisms of suppression of apoptosis. We have addressed the
novel possibility that the level of intracellular K+ regulates the apoptotic process by
controlling the activity of death enzymes. We show that K+, at normal intracellular levels,
inhibits both apoptotic DNA fragmentation and caspase-3 (CPP32)-like protease activation,
suggesting that intracellular K+ loss must occur early during apoptosis. Direct measurement
of K+ by inductively coupled plasma/mass spectrometry and flow cytometry indicates a major …
novel possibility that the level of intracellular K+ regulates the apoptotic process by
controlling the activity of death enzymes. We show that K+, at normal intracellular levels,
inhibits both apoptotic DNA fragmentation and caspase-3 (CPP32)-like protease activation,
suggesting that intracellular K+ loss must occur early during apoptosis. Direct measurement
of K+ by inductively coupled plasma/mass spectrometry and flow cytometry indicates a major …