[HTML][HTML] Reassessment of the role of FKBP38 in the Rheb/mTORC1 pathway
K Uhlenbrock, M Weiwad, R Wetzker, G Fischer… - FEBS letters, 2009 - Elsevier
K Uhlenbrock, M Weiwad, R Wetzker, G Fischer, A Wittinghofer, I Rubio
FEBS letters, 2009•ElsevierThe small G-protein Rheb regulates cell growth via the mTORC1 complex by incompletely
understood mechanisms. Recent studies document that Rheb activates mTORC1 via direct,
GTP-dependent interaction with the peptidyl-prolyl-cis/trans-isomerase FKBP38, which is
proposed to act as an inhibitor of mTORC1. We have conducted a comprehensive
biochemical characterization of the Rheb/FKBP38 interaction. Using three different in vitro
assays we did not detect an interaction between Rheb and FKBP38. Cell biological …
understood mechanisms. Recent studies document that Rheb activates mTORC1 via direct,
GTP-dependent interaction with the peptidyl-prolyl-cis/trans-isomerase FKBP38, which is
proposed to act as an inhibitor of mTORC1. We have conducted a comprehensive
biochemical characterization of the Rheb/FKBP38 interaction. Using three different in vitro
assays we did not detect an interaction between Rheb and FKBP38. Cell biological …
The small G-protein Rheb regulates cell growth via the mTORC1 complex by incompletely understood mechanisms. Recent studies document that Rheb activates mTORC1 via direct, GTP-dependent interaction with the peptidyl-prolyl-cis/trans-isomerase FKBP38, which is proposed to act as an inhibitor of mTORC1. We have conducted a comprehensive biochemical characterization of the Rheb/FKBP38 interaction. Using three different in vitro assays we did not detect an interaction between Rheb and FKBP38. Cell biological experiments illustrate that FKBP38 plays only a very minor, if any, role in mTORC1 activation. Our data document that FKBP38 is not the long-sought Rheb effector linking Rheb to mTORC1 activation. STRUCTURED SUMMARY: MINT-6946532: Ral (uniprotkb:P11233) binds (MI:0407) to Ha-Ras (uniprotkb:P01112) by pull down (MI:0096) MINT-6946500: RAF (uniprotkb:P04049) binds (MI:0407) to RHEB2 (uniprotkb:Q15382) by pull down (MI:0096) MINT-6946517: RAF (uniprotkb:P04049) binds (MI:0407) to Ha-Ras (uniprotkb:P01112) by pull down (MI:0096)
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