Inhibition of MHC class I-restricted antigen presentation by γ2-herpesviruses

PG Stevenson, S Efstathiou… - Proceedings of the …, 2000 - National Acad Sciences
Proceedings of the National Academy of Sciences, 2000National Acad Sciences
The γ-herpesviruses, in contrast to the α-and β-herpesviruses, are not known to inhibit
antigen presentation to CD8+ cytotoxic T lymphocytes (CTLs) during lytic cycle replication.
However, murine γ-herpesvirus 68 causes a chronic lytic infection in CD4+ T cell-deficient
mice despite the persistence of a substantial CTL response, suggesting that CTL evasion
occurs. Here we show that, distinct from host protein synthesis shutoff, γ-herpesvirus 68
down-regulates surface MHC class I expression on lytically infected fibroblasts and inhibits …
The γ-herpesviruses, in contrast to the α- and β-herpesviruses, are not known to inhibit antigen presentation to CD8+ cytotoxic T lymphocytes (CTLs) during lytic cycle replication. However, murine γ-herpesvirus 68 causes a chronic lytic infection in CD4+ T cell-deficient mice despite the persistence of a substantial CTL response, suggesting that CTL evasion occurs. Here we show that, distinct from host protein synthesis shutoff, γ-herpesvirus 68 down-regulates surface MHC class I expression on lytically infected fibroblasts and inhibits their recognition by antigen-specific CTLs. The viral K3 gene, encoding a zinc-finger-containing protein, dramatically reduced the half-life of nascent class I molecules and the level of surface MHC class I expression and was by itself sufficient to block antigen presentation. The homologous K3 and K5 genes of the related Kaposi's sarcoma-associated virus also inhibited antigen presentation and decreased cell surface expression of HLA class I antigens. Thus it appears that an immune evasion strategy shared by at least two γ-herpesviruses allows continued lytic infection in the face of strong CTL immunity.
National Acad Sciences