Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagen-derived peptides.

AE Postlethwaite, JM Seyer… - Proceedings of the …, 1978 - National Acad Sciences
AE Postlethwaite, JM Seyer, AH Kang
Proceedings of the National Academy of Sciences, 1978National Acad Sciences
The chemotactice response of human dermal fibroblasts of type I, II, and III human collagens
and collagen-derived peptides was quantitated by an in vitro assay. All three native human
collagens and constituent alpha chains can serve as chemoattractants for fibroblasts in vitro.
When type I, II, and III collagens were digested by bacterial collagenase, the resulting
peptides were also chemotactic. In addition, synthetic di-and tripeptides containing
hydroxyproline were also chemotactic for fibroblasts. Since collagen is degraded and …
The chemotactice response of human dermal fibroblasts of type I, II, and III human collagens and collagen-derived peptides was quantitated by an in vitro assay. All three native human collagens and constituent alpha chains can serve as chemoattractants for fibroblasts in vitro. When type I, II, and III collagens were digested by bacterial collagenase, the resulting peptides were also chemotactic. In addition, synthetic di- and tripeptides containing hydroxyproline were also chemotactic for fibroblasts. Since collagen is degraded and remodeled at sites of tissue injury and inflammation, these findings suggest that collagen and collagen-degradation peptides might function as chemotactic stimuli for fibroblasts in vivo and attract these cells to effect repair of damaged tissue.
National Acad Sciences