Increased expression of SAMHD1 in a subset of HIV-1 elite controllers
E Riveira-Munoz, A Ruiz, E Pauls… - Journal of …, 2014 - academic.oup.com
Journal of Antimicrobial Chemotherapy, 2014•academic.oup.com
Objectives SAMHD1 and the CDKN1A (p21) cyclin-dependent kinase inhibitor have been
postulated to mediate HIV-1 restriction in CD4+ cells. We have shown that p21 affects HIV
replication through its effect on SAMHD1. Thus, we aimed at evaluating the expression of
SAMHD1 and p21 in different HIV+ phenotypic groups. Patients and methods We evaluated
SAMHD1 and CDKN1A mRNA expression in CD4+ T cells from HIV+ individuals including
elite controllers (n= 12), individuals who control HIV without the need for antiretroviral …
postulated to mediate HIV-1 restriction in CD4+ cells. We have shown that p21 affects HIV
replication through its effect on SAMHD1. Thus, we aimed at evaluating the expression of
SAMHD1 and p21 in different HIV+ phenotypic groups. Patients and methods We evaluated
SAMHD1 and CDKN1A mRNA expression in CD4+ T cells from HIV+ individuals including
elite controllers (n= 12), individuals who control HIV without the need for antiretroviral …
Objectives
SAMHD1 and the CDKN1A (p21) cyclin-dependent kinase inhibitor have been postulated to mediate HIV-1 restriction in CD4+ cells. We have shown that p21 affects HIV replication through its effect on SAMHD1. Thus, we aimed at evaluating the expression of SAMHD1 and p21 in different HIV+ phenotypic groups.
Patients and methods
We evaluated SAMHD1 and CDKN1A mRNA expression in CD4+ T cells from HIV+ individuals including elite controllers (n = 12), individuals who control HIV without the need for antiretroviral treatment, viraemic progressors (n = 10) and HIV-1 seronegative healthy donors (n = 14). Immunological variables were measured by flow cytometry.
Results
We show that a subset of HIV+ elite controllers with lower T cell proliferation levels (Ki67+ cells) expressed higher SAMHD1 compared with healthy donors or viraemic progressors. Conversely, there was no difference in p21 expression before or after T cell activation with a bispecific CD3/CD8 antibody.
Conclusions
Our results suggest that SAMHD1 may play a role in controlling virus replication in HIV+ individuals and slow the rate of disease progression.
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