Mechanistic links between nonsense-mediated mRNA decay and pre-mRNA splicing in mammalian cells
F Lejeune, LE Maquat - Current opinion in cell biology, 2005 - Elsevier
F Lejeune, LE Maquat
Current opinion in cell biology, 2005•ElsevierNonsense-mediated mRNA decay (NMD) generally involves nonsense codon recognition
by translating ribosomes at a position∼ 25 nts upstream of a splicing-generated exon
junction complex of proteins. As such, NMD provides a means to degrade abnormal mRNAs
that encode potentially deleterious truncated proteins. Additionally, an estimated one-third of
naturally occurring, alternatively spliced mRNAs is also targeted for NMD. Given the
extraordinary frequency of alternative splicing together with data indicating that naturally …
by translating ribosomes at a position∼ 25 nts upstream of a splicing-generated exon
junction complex of proteins. As such, NMD provides a means to degrade abnormal mRNAs
that encode potentially deleterious truncated proteins. Additionally, an estimated one-third of
naturally occurring, alternatively spliced mRNAs is also targeted for NMD. Given the
extraordinary frequency of alternative splicing together with data indicating that naturally …
Nonsense-mediated mRNA decay (NMD) generally involves nonsense codon recognition by translating ribosomes at a position ∼25 nts upstream of a splicing-generated exon junction complex of proteins. As such, NMD provides a means to degrade abnormal mRNAs that encode potentially deleterious truncated proteins. Additionally, an estimated one-third of naturally occurring, alternatively spliced mRNAs is also targeted for NMD. Given the extraordinary frequency of alternative splicing together with data indicating that naturally occurring transcripts other than alternatively spliced mRNAs are likewise targeted for NMD, it is believed that mammalian cells routinely utilize NMD to achieve proper levels of gene expression.
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