Direct database searching with MALDI‐PSD spectra of peptides
PR Griffin, MJ Maccoss, RA Blevins… - Rapid …, 1995 - Wiley Online Library
Rapid Communications in Mass Spectrometry, 1995•Wiley Online Library
The analysis of matrix‐assisted laser desorption ionization post‐source decay (MALDI‐PSD)
mass spectra of peptides by using the cross‐correlation method for database searching is
illustrated. MALDI‐PSD mass spectra are shown to contain sufficient fragmentation
information to uniquely identify the correct amino acid sequence from large protein
databases (∼ 160000 entries). A search employing the MALDI‐PSD mass spectrum of a
phosphorylated peptide that correctly identifies the amino acid sequence and the site of …
mass spectra of peptides by using the cross‐correlation method for database searching is
illustrated. MALDI‐PSD mass spectra are shown to contain sufficient fragmentation
information to uniquely identify the correct amino acid sequence from large protein
databases (∼ 160000 entries). A search employing the MALDI‐PSD mass spectrum of a
phosphorylated peptide that correctly identifies the amino acid sequence and the site of …
Abstract
The analysis of matrix‐assisted laser desorption ionization post‐source decay (MALDI‐PSD) mass spectra of peptides by using the cross‐correlation method for database searching is illustrated. MALDI‐PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (∼160000 entries). A search employing the MALDI‐PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.
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