Cystathionine β-synthase (human)

JP Kraus - Methods in enzymology, 1987 - Elsevier
Publisher Summary Cystathionine β-synthase (L-serine hydro-lyase [adding homocysteine])
is a pyridoxal 5'-phosphate-dependent enzyme in the transsuifuration pathway of higher
eukaryotes which catalyzes the condensation of serine and homocysteine to form
cystathionine. This chapter describes a purification procedure starting with an" aged" liver
homogenate in which synthase molecules have been converted to the smaller, more active,
and more stable form. In assays of cystathionine β-synthase from extracts of cultured human …