A cell-based artificial antigen-presenting cell coated with anti-CD3 and CD28 antibodies enables rapid expansion and long-term growth of CD4 T lymphocytes

AK Thomas, MV Maus, WS Shalaby, CH June… - Clinical …, 2002 - Elsevier
We compared the ability of two genetically modified myeloid cells, K562 and U937, to serve
as artificial antigen-presenting cells (aAPC). Both aAPC were stably transfected with the low-
affinity Fcγ receptor CD32 (K32/U32 cells). K32 cells loaded with anti-CD3 and anti-CD28
Ab (K32/CD3/28) induced more rapid CD4 T-cell expansion than CD3/28-coated beads. In
contrast, U32/CD3/28 induced high levels of CD4 T-cell thymidine uptake but were unable to
sustain long-term T-cell expansion. K32 cells, but not U32 cells, loaded with anti-CD3 alone …