Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase
PM Bozeman, DB Learn, EL Thomas - Journal of immunological methods, 1990 - Elsevier
Conditions were optimized for measuring the activity of myeloperoxidase (MPO) and the
eosinophil peroxidase (EPO) with tetramethylbenzidine (TMB) as the substrate. Detergents
caused a small increase in the measured activity of the purified enzymes and were required
when isolated neutrophils or eosinophils were assayed. Sharp concentration optima were
observed with both ionic and non-ionic detergents. Activity was also influenced by halide
ions. Bromide or iodide caused up to a 7-fold increase in EPO activity and a 1.5-fold …
eosinophil peroxidase (EPO) with tetramethylbenzidine (TMB) as the substrate. Detergents
caused a small increase in the measured activity of the purified enzymes and were required
when isolated neutrophils or eosinophils were assayed. Sharp concentration optima were
observed with both ionic and non-ionic detergents. Activity was also influenced by halide
ions. Bromide or iodide caused up to a 7-fold increase in EPO activity and a 1.5-fold …