Detection of exosomal prions in blood by immunochemistry techniques
F Properzi, M Logozzi, H Abdel-Haq… - Journal of General …, 2015 - microbiologyresearch.org
F Properzi, M Logozzi, H Abdel-Haq, C Federici, L Lugini, T Azzarito, I Cristofaro, D di Sevo…
Journal of General Virology, 2015•microbiologyresearch.orgIn most forms of prion diseases, blood is infectious, but detection by immunochemistry
techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in
blood remains elusive. We developed a novel method for the detection of PrPTSE in blood
of prion-infected rodents based on the finding that PrPTSE is associated with plasma
exosomes. However, further purification of the exosomes on a sucrose gradient was
necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its …
techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in
blood remains elusive. We developed a novel method for the detection of PrPTSE in blood
of prion-infected rodents based on the finding that PrPTSE is associated with plasma
exosomes. However, further purification of the exosomes on a sucrose gradient was
necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its …
In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.
Microbiology Research