[HTML][HTML] Leptin-receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow

BO Zhou, R Yue, MM Murphy, JG Peyer, SJ Morrison - Cell stem cell, 2014 - cell.com
BO Zhou, R Yue, MM Murphy, JG Peyer, SJ Morrison
Cell stem cell, 2014cell.com
Studies of the identity and physiological function of mesenchymal stromal cells (MSCs) have
been hampered by a lack of markers that permit both prospective identification and fate
mapping in vivo. We found that Leptin Receptor (LepR) is a marker that highly enriches
bone marrow MSCs. Approximately 0.3% of bone marrow cells were LepR+, 10% of which
were CFU-Fs, accounting for 94% of bone marrow CFU-Fs. LepR+ cells formed bone,
cartilage, and adipocytes in culture and upon transplantation in vivo. LepR+ cells were Scf …
Summary
Studies of the identity and physiological function of mesenchymal stromal cells (MSCs) have been hampered by a lack of markers that permit both prospective identification and fate mapping in vivo. We found that Leptin Receptor (LepR) is a marker that highly enriches bone marrow MSCs. Approximately 0.3% of bone marrow cells were LepR+, 10% of which were CFU-Fs, accounting for 94% of bone marrow CFU-Fs. LepR+ cells formed bone, cartilage, and adipocytes in culture and upon transplantation in vivo. LepR+ cells were Scf-GFP+, Cxcl12-DsRedhigh, and Nestin-GFPlow, markers which also highly enriched CFU-Fs, but negative for Nestin-CreER and NG2-CreER, markers which were unlikely to be found in CFU-Fs. Fate-mapping showed that LepR+ cells arose postnatally and gave rise to most bone and adipocytes formed in adult bone marrow, including bone regenerated after irradiation or fracture. LepR+ cells were quiescent, but they proliferated after injury. Therefore, LepR+ cells are the major source of bone and adipocytes in adult bone marrow.
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