[HTML][HTML] Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation
AD Kohn, SA Summers, MJ Birnbaum… - Journal of Biological …, 1996 - ASBMB
Akt is a serine/threonine kinase that requires a functional phosphatidylinositol 3-kinase to be
stimulated by insulin and other growth factors. When directed to membranes by the addition
of a src myristoylation sequence, Akt becomes constitutively active. In the present studies,
the constitutively active Akt and a nonmyristoylated control mutant were expressed in 3T3-L1
cells that can be induced to differentiate into adipocytes. The constitutively active Akt
induced glucose uptake into adipocytes in the absence of insulin by stimulating …
stimulated by insulin and other growth factors. When directed to membranes by the addition
of a src myristoylation sequence, Akt becomes constitutively active. In the present studies,
the constitutively active Akt and a nonmyristoylated control mutant were expressed in 3T3-L1
cells that can be induced to differentiate into adipocytes. The constitutively active Akt
induced glucose uptake into adipocytes in the absence of insulin by stimulating …