Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
JD Dignam, RM Lebovitz, RG Roeder - Nucleic acids research, 1983 - academic.oup.com
JD Dignam, RM Lebovitz, RG Roeder
Nucleic acids research, 1983•academic.oup.comWe have developed a procedure for preparing extracts from nuclei of human tissue culture
cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of
extraction and assay have been optimized for maximum activity using the major late
promoter of adenovirus 2. The extract also directs accurate transcription initiation from other
adenovirus promoters and cellular promoters. The extract also directs accurate transcription
initiation from class III promoters (tRNA and Ad 2 VA).
cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of
extraction and assay have been optimized for maximum activity using the major late
promoter of adenovirus 2. The extract also directs accurate transcription initiation from other
adenovirus promoters and cellular promoters. The extract also directs accurate transcription
initiation from class III promoters (tRNA and Ad 2 VA).
Abstract
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
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