[HTML][HTML] Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus
BDE Raynal, TE Hardingham, JK Sheehan… - Journal of Biological …, 2003 - ASBMB
The macromolecular organization within saliva was investigated by tracer diffusion
measurements of fluorescent polystyrene microspheres by fluorescence recovery after
photobleaching using a confocal microscope (confocal-FRAP). There was a concentration-
dependent reduction in microsphere diffusion; this was much greater in the presence of
calcium (10 mm) and was reduced by the addition of EGTA (10 mm). These effects on tracer
diffusion showed that native saliva contained a macromolecular organization that was …
measurements of fluorescent polystyrene microspheres by fluorescence recovery after
photobleaching using a confocal microscope (confocal-FRAP). There was a concentration-
dependent reduction in microsphere diffusion; this was much greater in the presence of
calcium (10 mm) and was reduced by the addition of EGTA (10 mm). These effects on tracer
diffusion showed that native saliva contained a macromolecular organization that was …