Next-generation high-density self-assembling functional protein arrays
N Ramachandran, JV Raphael, E Hainsworth… - Nature …, 2008 - nature.com
Nature methods, 2008•nature.com
We developed a high-density self-assembling protein microarray, based on the nucleic acid
programmable protein array (NAPPA) concept, to display thousands of proteins that are
produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000
unique human cDNAs and obtained high yields of protein expression and capture with
minimal variation and good reproducibility. This method will enable various experimental
approaches to study protein function in high throughput.
programmable protein array (NAPPA) concept, to display thousands of proteins that are
produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000
unique human cDNAs and obtained high yields of protein expression and capture with
minimal variation and good reproducibility. This method will enable various experimental
approaches to study protein function in high throughput.
Abstract
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
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