[HTML][HTML] Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms (∗)
B Viollet, AM Lefrançois-Martinez, A Henrion… - Journal of Biological …, 1996 - ASBMB
The ubiquitous upstream stimulatory factor (USF) transcription factors encoded by two
distinct genes (USF1 and USF2) exist under the form of various dimers able to bind E-boxes.
We report the molecular cloning and functional characterization of USF2 isoforms,
corresponding to a 44-kDa subunit, USF2a, and a new 38-kDa subunit, USF2b, generated
by differential splicing. Using specific anti-USF antibodies, we define the different binding
complexes in various nuclear extracts. In vivo, the USF1/USF2a heterodimer represents over …
distinct genes (USF1 and USF2) exist under the form of various dimers able to bind E-boxes.
We report the molecular cloning and functional characterization of USF2 isoforms,
corresponding to a 44-kDa subunit, USF2a, and a new 38-kDa subunit, USF2b, generated
by differential splicing. Using specific anti-USF antibodies, we define the different binding
complexes in various nuclear extracts. In vivo, the USF1/USF2a heterodimer represents over …