Isolation of brain and spinal cord mononuclear cells using percoll gradients
PA Pino, AE Cardona - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Isolation of immune cells that infiltrate the central nervous system (CNS) during infection,
trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and
effector functions. Histochemical approaches are instrumental to determine the location of
the infiltrating cells and to analyze the associated CNS pathology. However, in-situ
histochemistry and immunofluorescent staining techniques are limited by the number of
antibodies that can be used at a single time to characterize immune cell subtypes in a …
trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and
effector functions. Histochemical approaches are instrumental to determine the location of
the infiltrating cells and to analyze the associated CNS pathology. However, in-situ
histochemistry and immunofluorescent staining techniques are limited by the number of
antibodies that can be used at a single time to characterize immune cell subtypes in a …