[HTML][HTML] The carboxyl-terminal domain of atypical protein kinase Cζ binds to ceramide and regulates junction formation in epithelial cells
G Wang, K Krishnamurthy, NS Umapathy… - Journal of Biological …, 2009 - ASBMB
Atypical protein kinase Cs (PKCs)(aPKCζ and λ/ι) have emerged as important binding
partners for ceramide, a membrane-resident cell signaling lipid that is involved in the
regulation of apoptosis as well as cell polarity. Using ceramide overlay assays with
proteolytic fragments of PKCζ and vesicle binding assays with ectopically expressed protein,
we show that a protein fragment comprising the carboxyl-terminal 20-kDa sequence of PKCζ
(C20ζ, amino acids 405–592) bound to C16: 0 ceramide. This sequence is not identical to …
partners for ceramide, a membrane-resident cell signaling lipid that is involved in the
regulation of apoptosis as well as cell polarity. Using ceramide overlay assays with
proteolytic fragments of PKCζ and vesicle binding assays with ectopically expressed protein,
we show that a protein fragment comprising the carboxyl-terminal 20-kDa sequence of PKCζ
(C20ζ, amino acids 405–592) bound to C16: 0 ceramide. This sequence is not identical to …