Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator
SP Lake, PD Bassett, A Larkins, J Revell, K Walczak… - Diabetes, 1989 - Am Diabetes Assoc
SP Lake, PD Bassett, A Larkins, J Revell, K Walczak, J Chamberlain, GM Rumford…
Diabetes, 1989•Am Diabetes AssocA new method is described for the large-scale purification of human pancreatic islets with a
discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator.
Fifteen human pancreases were processed, and after density-gradient centrifugation, a
mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and
contained in 430 μm mean vol. Viability of gradient-isolated islets was compared with that of
non-densitygradient islets (handpicked) and showed no difference in function. This …
discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator.
Fifteen human pancreases were processed, and after density-gradient centrifugation, a
mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and
contained in 430 μm mean vol. Viability of gradient-isolated islets was compared with that of
non-densitygradient islets (handpicked) and showed no difference in function. This …
A new method is described for the large-scale purification of human pancreatic islets with a discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator. Fifteen human pancreases were processed, and after density-gradient centrifugation, a mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and contained in 430 μm mean vol. Viability of gradient-isolated islets was compared with that of non-densitygradient islets (handpicked) and showed no difference in function. This technique allows isolation of intact, viable human islets of Langerhans of sufficient purity for potential human transplantation.
Am Diabetes Assoc