Haemopoietic colony formation (BFU-E, GM-CFC) during the development of pure red cell hypoplasia induced in the cat by feline leukaemia virus
NG Testa, D Onions, O Jarrett, F Frassoni, JF Eliason - Leukemia Research, 1983 - Elsevier
The GM-CFC assay for granulocyte-macrophage progenitors and the BFU-E and CFU-E
assay for early and late erythroid progenitors from cat bone marrow were characterized. GM-
CFC gave 59±4to118±6 colonies per 10 5 bone marrow cells using colony stimulating
factors (CSF) from cat, mouse or human sources. The CFU-E and BFU-E assays gave
114±7and58±7 colonies respectively with optimum doses of erythropoietin. Irradiated cat
bone marrow cells were good sources of CSF and of burst promoting activity for these …
assay for early and late erythroid progenitors from cat bone marrow were characterized. GM-
CFC gave 59±4to118±6 colonies per 10 5 bone marrow cells using colony stimulating
factors (CSF) from cat, mouse or human sources. The CFU-E and BFU-E assays gave
114±7and58±7 colonies respectively with optimum doses of erythropoietin. Irradiated cat
bone marrow cells were good sources of CSF and of burst promoting activity for these …