Recent studies argue for an important role for cholesterol in maintainingplasma membrane
heterogeneity and influencing a variety of cellular processes, including signaling, adhesion,
and permeability. Here, we document thattolerance-sensitive transitional immature B cells
maintain significantly lowermembrane unesterified cholesterol levels than mature-stage
splenic B cells. Inaddition, the relatively low level of cholesterol in transitional immature
Bcells impairs compartmentalization of their B cell receptor (BCR) intocholesterol-enriched …