The conversion of stearic to oleic acid by liver and yeast preparations
JB Marsh, AT James - Biochimica et Biophysica Acta, 1962 - Elsevier
1. Rat-liver homogenates, capable of converting stearic acid to oleic acid, were fractionated
by differential centrifugation. The isolated microsomes converted stearyl-CoA to oleate in the
presence of oxygen and reduced TPN. The supernatant solution contained the stearate-
activating enzymes. The oleic acid formed was isolated and degraded and shown to be the
natural Δ 9-isomer by gas-chromatographic identification of the radioactive azelaic acid
formed. 2. 2. Rat-liver homogenates, yeast cells, and cell-free yeast extracts which converted …
by differential centrifugation. The isolated microsomes converted stearyl-CoA to oleate in the
presence of oxygen and reduced TPN. The supernatant solution contained the stearate-
activating enzymes. The oleic acid formed was isolated and degraded and shown to be the
natural Δ 9-isomer by gas-chromatographic identification of the radioactive azelaic acid
formed. 2. 2. Rat-liver homogenates, yeast cells, and cell-free yeast extracts which converted …