A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression

MA Saleem, MJ O'Hare, J Reiser… - Journal of the …, 2002 - journals.lww.com
Recent molecular insights have established the podocyte as a key component of the
glomerular filtration barrier, and hence an important common pathway in proteinuric
diseases. A conditionally immortalized human podocyte cell line has been developed by
transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the
“permissive” temperature (33 C). After transfer to the “nonpermissive” temperature (37 C),
they entered growth arrest and expressed markers of differentiated in vivo podocytes …