Estimating podocyte number and density using a single histologic section
M Venkatareddy, S Wang, Y Yang, S Patel… - Journal of the …, 2014 - journals.lww.com
The reduction in podocyte density to levels below a threshold value drives
glomerulosclerosis and progression to ESRD. However, technical demands prohibit high-
throughput application of conventional morphometry for estimating podocyte density. We
evaluated a method for estimating podocyte density using single paraffin-embedded
formalin-fixed sections. Podocyte nuclei were imaged using indirect immunofluorescence
detection of antibodies against Wilms' tumor-1 or transducin-like enhancer of split 4. To …
glomerulosclerosis and progression to ESRD. However, technical demands prohibit high-
throughput application of conventional morphometry for estimating podocyte density. We
evaluated a method for estimating podocyte density using single paraffin-embedded
formalin-fixed sections. Podocyte nuclei were imaged using indirect immunofluorescence
detection of antibodies against Wilms' tumor-1 or transducin-like enhancer of split 4. To …