[HTML][HTML] A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry
We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus
nodules using an antibody that has previously reported IL-17A-positive neutrophils in
several inflammatory conditions. However, we could not detect IL-17A using a range of
alternative assays. Our data question the IL-17A antibody specificity and the ability of human
neutrophils to express IL-17A.
nodules using an antibody that has previously reported IL-17A-positive neutrophils in
several inflammatory conditions. However, we could not detect IL-17A using a range of
alternative assays. Our data question the IL-17A antibody specificity and the ability of human
neutrophils to express IL-17A.