[PDF][PDF] Distinct proteinase 3-induced cytokine patterns in Wegener's granulomatosis, Churg-Strauss syndrome, and healthy controls
U Fagin, E Csernok, A Müller, S Pitann… - Clinical and …, 2011 - clinexprheumatol.org
U Fagin, E Csernok, A Müller, S Pitann, J Fazio, K Krause, P Bremer, E Wipfler Freißmuth…
Clinical and Experimental Rheumatology-Incl Supplements, 2011•clinexprheumatol.orgObjective: To analyse whether a specific cytokine pattern is elicited in response to the
autoantigen proteinase 3 (PR3) in active Wegener's granulomatosis (WG). Methods: Six-
colour flow cytometry was used to analyse cytokine production and surface markers of the
total CD4+ T-cell population ex vivo and in PR3-stimulated T-cell lines of patients with active
PR3-ANCA-positive WG, PR3-ANCA-negative Churg-Strauss syndrome (CSS), and healthy
controls (HC). Results: The cytokine response of the total PB CD4+ T cell population was …
autoantigen proteinase 3 (PR3) in active Wegener's granulomatosis (WG). Methods: Six-
colour flow cytometry was used to analyse cytokine production and surface markers of the
total CD4+ T-cell population ex vivo and in PR3-stimulated T-cell lines of patients with active
PR3-ANCA-positive WG, PR3-ANCA-negative Churg-Strauss syndrome (CSS), and healthy
controls (HC). Results: The cytokine response of the total PB CD4+ T cell population was …
Abstract
Objective: To analyse whether a specific cytokine pattern is elicited in response to the autoantigen proteinase 3 (PR3) in active Wegener’s granulomatosis (WG).
Methods: Six-colour flow cytometry was used to analyse cytokine production and surface markers of the total CD4+ T-cell population ex vivo and in PR3-stimulated T-cell lines of patients with active PR3-ANCA-positive WG, PR3-ANCA-negative Churg-Strauss syndrome (CSS), and healthy controls (HC).
Results: The cytokine response of the total PB CD4+ T cell population was skewed towards distinct pro-inflammatory cytokine patterns in WG (Th1-type) and CSS (Th17, Th1-/Th2-type). Th2-type as well as Th17 cell populations including Th17/Th1, Th17/Th2 and Th22 cells were elicited in response to PR3 stimulation in WG. In contrast, CSS patients displayed a Th2-type dominated response following PR3 stimulation. Conclusion: These data suggest that the cytokine response of the total CD4+ T-cell population and PR3-specific cells is influenced by the underlying disorder.
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