[HTML][HTML] Cleavage of p21/WAF1/CIP1 by proteinase 3 modulates differentiation of a monocytic cell line: molecular analysis of the cleavage site
B Dublet, A Ruello, M Pederzoli, E Hajjar… - Journal of Biological …, 2005 - ASBMB
Proteinase 3 (PR3), also called myeloblastin, is involved in the control of myeloid cell
growth, but the underlying molecular mechanisms have not been elucidated. In U937/PR3,
stably transfected with PRCRSV/PR3 to overexpress PR3, PMA-induced p21 expression
was significantly decreased as compared with control U937, and this phenomenon was
reversed in the presence of the serine proteinase inhibitor, pefabloc. Conversely, when PR3
was inactivated by small interfering RNA, p21 protein was increased, and PMA-induced …
growth, but the underlying molecular mechanisms have not been elucidated. In U937/PR3,
stably transfected with PRCRSV/PR3 to overexpress PR3, PMA-induced p21 expression
was significantly decreased as compared with control U937, and this phenomenon was
reversed in the presence of the serine proteinase inhibitor, pefabloc. Conversely, when PR3
was inactivated by small interfering RNA, p21 protein was increased, and PMA-induced …