Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells
Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the
molecular mechanism and physiological function of bulk endocytosis remain elusive. In
pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash
photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent
process that is facilitated by enhanced extracellular Ca2+ entry and suppressed by the
inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied …
molecular mechanism and physiological function of bulk endocytosis remain elusive. In
pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash
photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent
process that is facilitated by enhanced extracellular Ca2+ entry and suppressed by the
inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied …
Abstract
Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca2+ entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
Oxford University Press