[HTML][HTML] Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation
OCT4 plays a critical role in maintaining stem cell pluripotency in a dose-dependent manner
by activating and repressing multiple downstream genes. The precise mechanism by which
OCT4 achieves these diverse biological functions remains unknown. In this report, we
identify and characterize 195 RKRKR as a nuclear localization signal responsible for its
localization in the nuclei and required for the transactivation of its target genes. Point
mutations within this motif yielded a mutant that localizes randomly throughout the cells and …
by activating and repressing multiple downstream genes. The precise mechanism by which
OCT4 achieves these diverse biological functions remains unknown. In this report, we
identify and characterize 195 RKRKR as a nuclear localization signal responsible for its
localization in the nuclei and required for the transactivation of its target genes. Point
mutations within this motif yielded a mutant that localizes randomly throughout the cells and …