[HTML][HTML] Genomic structure and promoter analysis of the human obese gene (∗)
The human gene encoding the homolog of the mouse obese (ob) gene was isolated and
partially characterized. The human ob gene consists of three exons and two introns and
spans about 18 kilobase pairs (kb), encoding a 3.5-kb cDNA. A 3-kb 5′-flanking region of
the gene was cloned and transient transfection assay with luciferase reporter confirmed the
promoter activity in differentiated F442-A adipocytes. Potential regulatory elements are
discussed in this report.
partially characterized. The human ob gene consists of three exons and two introns and
spans about 18 kilobase pairs (kb), encoding a 3.5-kb cDNA. A 3-kb 5′-flanking region of
the gene was cloned and transient transfection assay with luciferase reporter confirmed the
promoter activity in differentiated F442-A adipocytes. Potential regulatory elements are
discussed in this report.