[HTML][HTML] Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal region interferes with ras-dependent activation of Raf-1
CD Hu, K Kariya, G Kotani, M Shirouzu… - Journal of Biological …, 1997 - ASBMB
Raf-1 is a major downstream effector of mammalian Ras. Binding of the effector domain of
Ras to the Ras-binding domain of Raf-1 is essential for Ras-dependent Raf-1 activation.
However, Rap1A, which has an identical effector domain to that of Ras, cannot activate Raf-
1 and even antagonizes several Ras functionsin vivo. Recently, we identified the cysteine-
rich region (CRR) of Raf-1 as another Ras-binding domain. Ha-Ras proteins carrying
mutations N26G and V45E, which failed to bind to CRR, also failed to activate Raf-1. Since …
Ras to the Ras-binding domain of Raf-1 is essential for Ras-dependent Raf-1 activation.
However, Rap1A, which has an identical effector domain to that of Ras, cannot activate Raf-
1 and even antagonizes several Ras functionsin vivo. Recently, we identified the cysteine-
rich region (CRR) of Raf-1 as another Ras-binding domain. Ha-Ras proteins carrying
mutations N26G and V45E, which failed to bind to CRR, also failed to activate Raf-1. Since …