Characterization of virus‐specific cytotoxic T cell clones from allogeneic bone marrow chimeras

H Pircher, J Baenziger, M Schilham… - European journal of …, 1987 - Wiley Online Library
H Pircher, J Baenziger, M Schilham, T Sado, H Kamisaku, H Hengartner, RM Zinkernagel
European journal of immunology, 1987Wiley Online Library
We established several H‐2‐restricted lymphocytic choriomeningitis virus (LCMV)‐specific
cytotoxic T cell clones from spleens of virus‐primed C57BL/6 or C57BL/10 (H‐2b) and B10.
BR (H‐2k) mice and from allogeneic C57BL/10→ B10. BR and B10. BR→ C57BL/10 bone
marrow chimeras. Two T cell clones of H‐2b origin and restricted to H‐2b, 3 of H‐2k origin
and restricted to H‐2k were compared with two clones each derived from the two types of
chimeras. Their surface phenotype was found to be Lyt‐2+, L3/T4− and KJ16‐133+(2 of 9) …
Abstract
We established several H‐2‐restricted lymphocytic choriomeningitis virus (LCMV)‐specific cytotoxic T cell clones from spleens of virus‐primed C57BL/6 or C57BL/10 (H‐2b) and B10.BR (H‐2k) mice and from allogeneic C57BL/10→B10.BR and B10.BR→C57BL/10 bone marrow chimeras. Two T cell clones of H‐2b origin and restricted to H‐2b, 3 of H‐2k origin and restricted to H‐2k were compared with two clones each derived from the two types of chimeras. Their surface phenotype was found to be Lyt‐2+, L3/T4 and KJ16‐133+ (2 of 9). Clones from chimeras expressed bone marrow donor H‐2 and are restricted to the recipient H‐2.
H‐2k‐restricted clones were all specific for Kk whereas all H‐2b‐restricted clones were specific for Db. These restriction specificities could be further defined by the blocking activity of various monoclonal anti‐H‐2 antibodies. Interestingly the anti‐H‐2Db antibodies blocked the restricted virus‐specific killing activity of the clones derived B10.BR→C57BL/10 chimeras much more effectively than the activity of the clones derived from conventional H‐2b mice.
The various clones differed with respect to their fine specificity for LCMV strains. The 3 clones of conventional B10.BR origin only recognized LCMV‐WE but not LCMV‐Armstrong, Aggressive or Docile; H‐2b‐restricted conventional clones recognized target cells infected with all LCMV strains except LCMV‐UBC‐Docile; the T cell clones from the bone marrow chimeras recognized with one exception all LCMV strains tested.
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