[HTML][HTML] Nuclear receptor-mediated repression of human cholesterol 7α-hydroxylase gene transcription by bile acids
W Chen, E Owsley, Y Yang, D Stroup… - Journal of lipid …, 2001 - ASBMB
Hydrophobic bile acids strongly repressed transcription of the human cholesterol 7α-
hydroxylase gene (CYP7A1) in the bile acid biosynthetic pathway in the liver. Farnesoid X
receptor (FXR) repressed CYP7A1/Luc reporter activity in a transfection assay in human
liver-derived HepG2 cells, but not in human embryonic kidney (HEK) 293 cells. FXR-binding
activity was required for bile acid repression of CYP7A1 transcription despite the fact that
FXR did not bind to the CYP7A1 promoter. FXR-induced liver-specific factors must be …
hydroxylase gene (CYP7A1) in the bile acid biosynthetic pathway in the liver. Farnesoid X
receptor (FXR) repressed CYP7A1/Luc reporter activity in a transfection assay in human
liver-derived HepG2 cells, but not in human embryonic kidney (HEK) 293 cells. FXR-binding
activity was required for bile acid repression of CYP7A1 transcription despite the fact that
FXR did not bind to the CYP7A1 promoter. FXR-induced liver-specific factors must be …