Increased circulating Lin−/lowCD33+HLA‐DR− myeloid‐derived suppressor cells in hepatocellular carcinoma patients
P Shen, A Wang, M He, Q Wang… - Hepatology …, 2014 - Wiley Online Library
P Shen, A Wang, M He, Q Wang, S Zheng
Hepatology Research, 2014•Wiley Online LibraryAim Myeloid‐derived suppressor cells (MDSC) can be induced or expanded in tumor‐
bearing mice and cancer patients. The frequency of MDSC denoted here as L in−/low CD
33+ HLA‐DR− was investigated in hepatocellular carcinoma (HCC) patients. The clinical
relevance of MDSC and patients' characteristics were examined. Also, MDSC‐related
immune regulatory pathways in these patients were discussed. Methods The quantity of
MDSC was tested in peripheral blood of patients with HCC (n= 63) and healthy donors (n …
bearing mice and cancer patients. The frequency of MDSC denoted here as L in−/low CD
33+ HLA‐DR− was investigated in hepatocellular carcinoma (HCC) patients. The clinical
relevance of MDSC and patients' characteristics were examined. Also, MDSC‐related
immune regulatory pathways in these patients were discussed. Methods The quantity of
MDSC was tested in peripheral blood of patients with HCC (n= 63) and healthy donors (n …
Aim
Myeloid‐derived suppressor cells (MDSC) can be induced or expanded in tumor‐bearing mice and cancer patients. The frequency of MDSC denoted here as Lin−/lowCD33+HLA‐DR− was investigated in hepatocellular carcinoma (HCC) patients. The clinical relevance of MDSC and patients' characteristics were examined. Also, MDSC‐related immune regulatory pathways in these patients were discussed.
Methods
The quantity of MDSC was tested in peripheral blood of patients with HCC (n = 63) and healthy donors (n = 56). The expressions of interferon (IFN)‐γ, vascular endothelial growth factor (VEGF), cyclooxygenase (COX)‐2, matrix metalloproteinase (MMP)‐13, nitric oxide synthase (NOS)‐2 and arginase (ARG)‐1 were analyzed. Co‐culturing with anti‐CD3/CD28‐stimulated T lymphocytes was used to determine the suppressive effect of MDSC on the T lymphocytes.
Results
Patients with treatment‐naive HCC had an increased subpopulation of Lin−/lowCD33+HLA‐DR− cells in the peripheral blood mononuclear cells (PBMC) with characteristics of MDSC and associated to the stage (P = 0.0004). Patients with splenomegaly had a higher frequency of circulating MDSC. Also, COX‐2, MMP‐13 and VEGF were expressed differently associated with the alteration of MDSC.
Conclusion
Our study provides evidence showing an increased population of Lin−/lowCD33+HLA‐DR− MDSC in the peripheral blood of HCC patients. Our data also suggest that MMP‐13 and COX‐2 in PBMC may play a new important role companied with MDSC in HCC patients.
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