Immunofluorescent detection of two thymidine analogues (CldU and IdU) in primary tissue
AH Tuttle, MM Rankin, M Teta, DJ Sartori… - JoVE (Journal of …, 2010 - jove.com
Accurate measurement of cell division is a fundamental challenge in experimental biology
that becomes increasingly complex when slowly dividing cells are analyzed. Established
methods to detect cell division include direct visualization by continuous microscopy in cell
culture, dilution of vital dyes such as carboxyfluorescein di-aetate succinimidyl ester (CFSE),
immuno-detection of mitogenic antigens such as ki67 or PCNA, and thymidine analogues.
Thymidine analogues can be detected by a variety of methods including radio-detection for …
that becomes increasingly complex when slowly dividing cells are analyzed. Established
methods to detect cell division include direct visualization by continuous microscopy in cell
culture, dilution of vital dyes such as carboxyfluorescein di-aetate succinimidyl ester (CFSE),
immuno-detection of mitogenic antigens such as ki67 or PCNA, and thymidine analogues.
Thymidine analogues can be detected by a variety of methods including radio-detection for …