Selenium and amino acid composition of selenoprotein P, the major selenoprotein in rat serum.
R Read, T Bellew, JG Yang, KE Hill, IS Palmer… - Journal of Biological …, 1990 - ASBMB
Selenoprotein P is the second plasma selenoprotein to be purified. It is a glycoprotein and
has been shown to be distinct from plasma glutathione peroxidase. This study characterizes
selenoprotein P further. Deglycosylation of the protein shifts its migration on sodium dodecyl
sulfate-polyacrylamide gel electrophoresis from Mr 57,000 to Mr 43,000, indicating it has a
substantial carbohydrate component. Measurement of selenium indicates a selenium
content of 7.5+/-1.0 atoms/molecule based on a polypeptide weight of 43,000. Amino acid …
has been shown to be distinct from plasma glutathione peroxidase. This study characterizes
selenoprotein P further. Deglycosylation of the protein shifts its migration on sodium dodecyl
sulfate-polyacrylamide gel electrophoresis from Mr 57,000 to Mr 43,000, indicating it has a
substantial carbohydrate component. Measurement of selenium indicates a selenium
content of 7.5+/-1.0 atoms/molecule based on a polypeptide weight of 43,000. Amino acid …