Postcolumn fluorometric detection system for liquid chromatographic analysis of amino and imino acids using o-phthalaldehyde/N-acetyl-L-cysteine reagent
M Fujiwara, Y Ishida, N Nimura, A Toyama… - Analytical …, 1987 - Elsevier
M Fujiwara, Y Ishida, N Nimura, A Toyama, T Kinoshita
Analytical biochemistry, 1987•ElsevierA high-performance liquid chromatographic system for the determination of amino acid and
imino acid was developed using a two-step reaction with sodium hypochlorite and o-
phthalaldehyde/N-acetyl-l-cysteine (OPTA AcCys) reagent. This reagent improved the
sensitivity in the analysis of proline presumably because the fluorophore is more stable to
hypochlorite which has been used for the oxidative cleavage of the imino linkage. The use of
OPTA AcCys facilitated the detection of imino acids at the same concentration level as that of …
imino acid was developed using a two-step reaction with sodium hypochlorite and o-
phthalaldehyde/N-acetyl-l-cysteine (OPTA AcCys) reagent. This reagent improved the
sensitivity in the analysis of proline presumably because the fluorophore is more stable to
hypochlorite which has been used for the oxidative cleavage of the imino linkage. The use of
OPTA AcCys facilitated the detection of imino acids at the same concentration level as that of …
A high-performance liquid chromatographic system for the determination of amino acid and imino acid was developed using a two-step reaction with sodium hypochlorite and o-phthalaldehyde/N-acetyl-l-cysteine (OPTA AcCys ) reagent. This reagent improved the sensitivity in the analysis of proline presumably because the fluorophore is more stable to hypochlorite which has been used for the oxidative cleavage of the imino linkage. The use of OPTA AcCys facilitated the detection of imino acids at the same concentration level as that of amino acids. The detection limit for all the amino and imino acids was a few picomoles. This detection system, together with cation-exchange chromatographic separation, was applied to the determination of amino and imino acids in biological samples.
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